YSI 2950 at the Cutting Edge in Pancreatic Cancer Research

The YSI 2950 Biochemical Analyser is rapidly becoming an invaluable tool in many areas of cutting-edge clinical research. It’s application in oncology, the study of tumours, is particularly note-worthy because it is aiding in work that really does help save lives.

Pancreatic ductal adenocarcinoma (PDAC) is a deadly cancer, one with fast onset and a depressingly low survival rate.

One approach for treatment involves targeting oncogene-driven signalling pathways and this has had some success in other equally devastating diseases. However, with PDAC, despite some success in shrinking the tumours themselves, the relapse frequency rate indicates that a significant
fraction of tumour cells are surviving the shut down of their oncogene-driven signalling pathways.

An important paper published in the prestigious journal Nature has recently illustrated how scientists are making full use of the YSI 2950 in their investigation of these surviving cells.

Specifically the metabolic activity of those cells that survived the ‘oncogene ablation’ was analysed using the YSI 2950. Measured numbers of these dormant tumour cells were plated out, in triplicate, in a 96-well plate in 200 ml of complete steam cell medium. After 24 hours incubation the medium was analysed for lactate and glucose levels with the YSI 2950, using unconditioned medium as a reference.

This analysis contributed to the important finding that these surviving cells have a decreased reliance on glycolysis for cellular energetic and stronger reliance on mitochondrial respiration. This makes them sensitive to oxidative phosphorylation inhibitors, which means that these then become a possible treatment to prevent tumour recurrence.

The speed accuracy and reliability of the YSI 2950 Biochemical Analyser is exactly appropriate for this level of research. In addition to glucose and lactate many other chemistries may be measured, including glutamine, glutamate, glucose, dextrose, sucrose, xylose, lactate, lactose, methanol,
ethanol and hydrogen peroxide.

Up to 6 analytes can be measured at one time, depending upon the nature and number of modules you require.

Date added: 2015-05-25 14:29:55