Centrifugal Concentrator Provides Versatile Ultrafiltration

Sartorius Stedim Biotech now offers a range of twelve different centrifugal concentrators, with five different membrane types, to cover a variety of research needs. Centrifugal concentrators, otherwise known as spin filters, centrifugal ultrafiltration devices or spin devices are an elegant, straightforward way of concentrating biological samples such as antibodies, antigens, enzymes and nucleic acids.

They are equally effective for the purification of macromolecular components from cell lysates and tissue culture material and an useful and speedy alternative to dialysis for desalting of biomolecules and buffer exchange. Whatever their eventual use, centrifugal concentrators work on a simple principle: differential centrifugal separation through an anisotropic membrane.

For ‘concentration’ the sample solution is placed above the membrane in the device tube and centrifugal force results in the accumulation of filtrate and smaller molecules (below the membrane cut-off size) in the lower portion of the tube. Adding a retentate vial, and inverting the device, allows the concentrated sample to be recovered, with minimal adsorption losses, following a brief centrifugation. Similarly the filtrate will have been cleaning of larger molecules.

Sartorius Stedim Biotech provide an extensive range of centrifugal concentrators for many different uses. They come in both reusable and disposable models and will handle sample volumes from 100 µl up to 100 ml. Membrane types available include polyethersulfone (PES), Hydrosart®, regenerated cellulose (RC), cellulose triacetate (CTA), polyethersulfone (PES) and cellulose triacetate (CTA). This provides a wide range of molecular weight ‘cut offs’, and sample volumes, to match your individual research requirements.

Date added: 2015-05-25 12:02:17