YSI Biochemistry Analyser for Glucose and Xylose Analysis During Fermentation

Further evidence for the efficacy of the YSI Biochemistry Analyser in providing a rapid, easy-to-use analytical alternative to High-performance Liquid Chromatography (HPLC) has come from an investigation of corn stover.

Stover is the name given to the husk, leaf and cob residue that remains in the field after the harvest of maize cereal grain. The name’s not actually anything to with “what’s over” after harvesting, but is actually derived from the French noun “estover”, meaning ‘to be necessary’.

Apart from its use as fodder, stover has enormous potential for biofuel production. It’s been estimated that corn stover and similar ‘ligno-cellulosic’ plants could provide 1.3 billion tons of raw materials, which could make enough fuel for the next 50 years.

This is obviously an important area of research then and one where speedy and efficient measuring could produce real financial savings. The progress of biofuel fermentation is usually monitored using HPLC to measure the levels of glucose and xylose. This is accurate, but significant sample pretreatment is required and, at 30 minutes plus, the analytical cycle time is not ideal. Alternatives would be welcomed.

In a comparative study of filtered corn stover, glucose and xylose were both sampled from a laboratory bioethanol fermentation procedure over a 48-hour period using HPLC and the immobilised enzyme technology of YSI’s Biochemistry Analyser.

A YSI 2900 Biochemistry Analyser was configured with glucose oxidase and pyranose oxidase immobilised enzyme electrodes. In this configuration the YSI 2900 can produce results for both analytes in under a minute.

The results demonstrate a strong, positive correlation of the two analytical methods showing that the YSI 2900 Biochemistry Analyser is as efficient while being significantly faster and easier to use. As far as fermentation analysis goes it seems as if the YSI 2900 Biochemistry Analyser has proved it may well be “estover”, as the French might say.

Date added: 2015-05-25 15:49:25