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Multiphoton microscopy at the highest level

Nikon offers the ultimate equipment for confocal imaging and multiphase microscopy.

The A1R MP is a unique multiphase imaging system with a high resolution galvanometer scanner and high speed resonant scanner capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode.

Four channel non-descanned detectors feature higher sensitivity, reduced dark current and broad spectral range, which allows for real-time unmixing of closely spaced probes to give accurate, high-contrast spectral imaging. This is vital in multiphase microscopy because of the overlap of emission spectra of poles and autofluorescence, which is usually unavoidable when using a single laser line.

Imaging up to 420 fps

The exclusive Nikon resonant scanner is capable of imaging a wide area at a far higher speed than non-resonant scanners, making 420 fps imaging a reality. The NDD*1 for multiphase microscopy means it is now possible to image fast and deep through even the thickest specimens, while Nikon's optical pixel clock system means more stable and evenly illuminated  imaging even at high speeds.

Deep imaging with high sensitivity

Nikon's specially-developed four-channel NDD for multiphase microscopy means images can be captured deep within a specimen. A high sensitivity detector placed close to the back of the aperture of the objective where image formation takes place enables fine and detailed images.

This set-up boosts detection efficiency for scattered fluorescence, enabling the capture of signals from inside a living organism far more clearly and more reliably with higher S/N and fewer aberrations.

Sharper, brighter imaging

New water immersion objectives give improved chromatic aberration correction across a wide wavelength and high transmission from near-ultraviolet to near-infrared. This is made possible by Nikon's proprietary Nano Crystal Coat. Furthermore, the confocal microscope also offers high-contrast imaging with outstanding brightness and resolution.

High-speed, high-precision unmixing

As well as spectral detection and unmixing by the 32-channel detector, unmixing by the four-channel NDD for multiphase microscopy is also a feature. Images captured with a resonant scanner can be unmixed, meaning clear, high-contrast images from areas deep inside the specimen can be captured at ultrahigh speed.

Multiphoton laser beam alignment

On alteration of the multiphase laser wavelength or group dispersion recommendation, the multiphase laser bean positional pointing can drift. This means uneven intensity of images or misalignment of fluorescence images produced by a visible light laser and multiphase laser.  Nikon's confocal microscope overcomes this problem with an auto laser alignment function which corrects multiphase laser misalignments in a single click, removing difficulty and danger when aligning the laser beam.

Date added: 2015-05-25 11:32:02