Mouse Embryonic Fibroblasts for Stem Cell Culture

Amsbio (AMS Biotechnology) are a leading supplier of reagents and products for the Life Sciences. Appreciating the role of stem cell research in so much important medical research today, amsbio have consigned a significant commitment to the supply of embryonic and adult stem cells, stem cell culturing, differentiation and assaying products as well as adressing the problems of stem cell storage. The production of Mouse Embryonic Fibroblasts (MEFS) and Newborn Human Foreskin Fibroblasts (NuFFs) has been an integral part of this development plan.

Mouse Embryonic Fibroblast cells have a vital role in stem cell research where they function as ‘feeder cells' for different lines, providing a substratum that allows cells, such as human embryonic stem cells, to be cultured. Mouse Embryonic Fibroblasts produce a number of components e.g. collagens, glycosaminoglycans, elastic and reticular fibres and cytokines, that help produce the correct conditioned environment for certain stem cells, such as human embryonic cells (hESC) to be maintained. Lacking the optimal culture conditions many embryonic stem cells, such as hESCs will rapidly differentiate.

Newborn Human Foreskin Fibroblasts (NuFFs) have a similar application to Mouse Embryonic Fibroblasts for the maintenance of undifferentiated ESC and iPSC lines. An iPSC being an induced pluripotent cell artificially obtained from an adult somatic cell via specific gene activation, rather than having natural pluripotency.

The two sets of feeder cells from amsbio come either as treated or untreated cryoprotected and frozen samples; standard concentration 4-5 million per vial or higher density 7-8 million cells per vial. Treatment is by irradiation or mitomycin to arrest growth of the feeder cell layer.

Samples are thoroughly tested to maintain a consistent performance with every batch, for post-thaw viability and also to ensure that there is no chance of contamination by pathogens. Mouse Embryonic Fibroblasts and Newborn Human Foreskin Fibroblasts supplied by amsbio save you time and trouble allowing you to apply yourself to the research which really counts.

Date added: 2015-05-25 11:58:55