Measuring L-lactate in Cooked Meats – Warning May Contain Spam!

‘Spam, spam, spam, spam. Spam, spam, spam, spam. Spam, wonderful Spam!’

The Spam song from Monty Python’s Flying Circus is as recognisable to people of a certain generation as is the product itself. Spam, which may or may not stand for ‘Shoulder of Pork and Ham’, is one of a number of cooked meats that are often called luncheon meats or cold cuts.

These vital components of many a quick snack contain sophisticated and complex matrices and, of course, like other pre-cooked foodstuffs must be rigorously analysed and maintained in prime condition. Lactic acid is present in many of these meat products, as well as cheese and yoghurt, where it is formed by natural fermentation.

It is also used as a preservative, flavouring agent and pH regulator. Often added as the sodium or potassium salt, it has a wide role in extending the shelf life of poultry and fish as well as red meats, by helping to control dangerous bacteria.

The YSI 2900 Series Biochemistry Analyser can give you accurate measurement of L-lactate levels, even from a complex matrix, like a luncheon meat, in one minute or less. The proprietary enzyme technology developed by YSI is virtually unaffected by pH, density, the presence of reducing substances, colour or turbitity and what’s more the ‘recipe’ for producing your results is remarkably straight forward!

- Simply take your luncheon meat, cut into 1 cm squares and weigh.

- Blend your meat with about a cup of water (deionised) for 5 minutes. Dilute to half a litre with more water.

- Inject the sample into the chamber of the YSI 2900 Series Biochemistry Analyser and the L-Lactate diffuses into the L-lactate oxidase containing membrane.

Oxidation of the L-Lactate occurs to produce hydrogen peroxidase and pyruvate. Hydrogen peroxidase is detected at the surface of the platinum electrode amperometrically with current flow directly proportional to the concentration of hydrogen peroxidase and the initial L-lactate concentration.

Couldn’t be simpler, precise accurate results in under a minute! And of course the YSI 2900 Series Biochemistry Analyser is suitable for measuring L-lactate in dairy products, beverages, confectionery, ready meals, bakery products and all sorts of other foodstuffs. Plus, because of its modular design, the 2900 series can measure up to six different chemistries with three separate sensor modules.  Something to sing about!

Date added: 2015-05-25 12:23:39