Laser Scanning Confocal Microscope

The Olympus FluoView FV1000MPE multiphoton laser scanning microscope allows outstanding fluorescence imaging even into deep regions of samples.

It utilises pulsed IR lasers in combination with long working distance objectives to image hundreds of microns into living tissues, giving the highest penetration depths available.

As well as allowing deep imaging, the long wavelength IR light has a lower energy content, therefore reducing phototoxicity and the risk of photodamage and making long range and time-lapse studies a reality.

Multiphoton objective

Olympus designed the multiphase objective lens XLPLN 25x WMP to take into account all MPE-related requirements.

With high transmission in both the IR and visible light range, it allows as much light as possible to get to the sample.

With an NA of 1.05 the excitation volume is very small and the enhanced large field-of-view allows efficient collection of the scattered fluorescence signal. In-vivo imaging is further boosted by the working distance of 2 mm and a ceramic tip.

Furthermore, the correction collar compensates for spherical aberrations when imaging deep within a specimen.

Femtosecond pulsed IR laser

The femtosecond pulsed IR laser with pulse-recommendation (negative chirp) greatly reduces stretching of laser pulses and almost completely restores the original pulse width.

A Mai Tai DeepSee or Chameleon Vision can be adapted to the FV1000MPE and both exist as special Olympus versions, designed to give the perfect combination of laser and multiphoton microscope.

Motorised beam expander optics

A small excitation volume and guiding as much IR light to the sample as possible are both crucial, meaning the back focal aperture should be filled by the excitation laser beam. But having the beam larger than the back focal aperture would mean a loss of excitation laser light.

The Olympus laser scanning confocal microscope ensures that for any objective and wavelength used, the size of the IR beam fits to the backfocal aperture of the objective.

Non-descanned detector system

The FV1000MPE gives efficient detection with minimal loss by using a non-descanned (NDD) system close to the specimen, equipped with two or four PMT detectors in the reflective pathway.

Furthermore, the emitted fluorescence can be collected via a special condensor lens in the transmission pathway and directed onto two PMT detectors. This is  ideal for SHG imaging.

Dichromatic mirrors in the light path separate the emitted fluorescence.

Both the mirrors and filters can be changed easily according to the sample’s fluorescence emission characteristics.

Simultaneous MPE imaging and MPE stimulation

The laser scanning confocal microscope can incorporate two laser scanners for simultaneous fluorescence imaging and independent laser light sitmulation.

Any area of the sample can be specified for stimulation and scanning independently, with a high level of control over timing, duration and intensity. 

This opens up applications such as FRAP, FLIP, photoactivation, photoconversion, uncaging, laser ablation and many others, deep into living tissue

The FV1000MPE laser scanning confocal microscope provides cutting-edge technology in many areas of scientific research and is available in three configurations. It is ideal for situations that require in vivo observation, deep tissue penetration, minimised photodamage/photo toxicity and reduced photobleaching.

Date added: 2011-04-15 09:28:11