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Glutamine And Glutamate Analysis

An article explaning the procedure of the YSI 2900 series Biochemistry Analyzer in it's advanced measurements of both Glutamine and Glutamate in complex matrices.  Results are not stifled by reducing substances, density, pH, colour or turbidity.

The ability to measure both glutamine and glutamate occurs when the glutamate causes interference with the glutamine analysis. Once a sample has been injected in to the sample chamber, the glutamine becomes diffused to the glutamate membrane. 

Once diffused, the glutamine is exposed to glutaminase and glutamate oxidase which oxidises the glutamine to hydrogen peroxide, α-ketoglutarate and ammonia. Amperometrically the hydrogen peroxide is identified at the platinum electrode surface. With the current flow at the electrode measuring directly proportional to the hydrogen peroxide, accurate measurements fro glutamate can be taken.

While the glutamine results are achieved similarly as the glutamate is oxidised at both glutamate and glutamine membranes causing hydrogen peroxide, α-ketoglutarate and ammonia.

The YSI 2900 series Biochemistry Analyzer is able to calculate the exact glutamine concentration by subtracting the glutamate response from the glutamine response. In doing so, the YSI 2900 series Biochemistry Analyzer is able to measure both concentrations simultaneously.

Please click on the GET PDF icon to your right to view the associated application note.

Date added: 2015-05-25 11:35:33