Glass For Microbiology From DURAN
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Date Added: 27th August 2011
You would have thought that the capacity to allow light to pass was a rather basic function of clear glass - well fortunately it is. However, when it comes to light microscopy mere transparency is not enough, as Duran illustrate in their manufacturing of microscope slides.
Considerable attention has been paid to the flatness (planarity) and uniformity of thickness (1.0±0.05 mm) of these slides and ensuring that they remain free from surface faults, steaks and bubbles; vital for good microscopy. Similar attention has been paid to the properties of Duran cover slips and petri dishes, eliminating distortion during the examination of growing cultures in microbiology and during tissue culture.
Petri dishes are also manufactured to promote a uniform distribution of Agar, in borosilicate (hydrolytical class I), or in soda-lime (hydrolytical class III) versions. The high thermal-shock resistance of the glass makes it very suited to autoclaving and sterilisation processes and its inertness helps preclude detrimental ion exchange between glass and contents.
This is obviously also an essential attribute for the Duran range of culture bottles and flasks for microbiology. Bottles come in 50, 100 and 300 ml capacities and are straight rimmed for Kapsenberg caps. Fernbach type microbiology culture flasks have a large 20cm diameter flat bottom to allow a uniform culture thickness and come with a straight neck or a beaded rim.
The Erlenmeyer-style glass microbiology culture flasks, which are more conical in shape, and ideally suited for media optimisation (shaking) tests. These flasks come either with a straight neck, suitable for a metal cap, or with a DIN thread. The threaded version allows a membrane cap to be used which greatly reduces the risk of contamination, while producing a more reproducible degree of gas exchange compared with cotton wadding.
A similar threaded option is available on the Duran baffled, glass microbiology culture flask. Because oxygen intake is often the limiting factor in cell culture growth on a vibrating board, four baffles have been incorporated into the flask design producing turbulent flow within and increasing the surface area of the nutrient mixture available for gas exchange.
Special manufacturing processes ensure consistency of production, and hence results, and an increased wall thickness gives a longer product life. Altogether the Duran range for microbiology, and associated usage, simply proves there is more to glass than meets the eye.