Fluorescence Microscope For Mammalian Cell Counting
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Date Added: 10th July 2012
When you look at the NucleoCounter it's basically a fluorescence microscope that has been integrated. All the essential parts to this piece contain an aluminum profile. It also has the computing part used for image analysis that can count cells in any sample. However, there are other features as well like; a printer port, USB port, DC input voltage port, keyboard, and display.
LED's or Light Emitting Diodes are part of the excitation source that offers high stability and durability that allows them to last virtually forever. All the wavelengths are removed by a filter that the excitation light possesses. The light that won't be affected is the green light since it illuminates the sample. The red fluorescence light is the only one allowed to pass through the emission filter.
These are hard-coated interference filters that have unbelievable blocking capabilities. Once the red fluorescence passes through it focuses onto the detector. The lenses are identical and each of them is fastened securely. While these are beneficial, the key is the low magnification of the microscope. The effects of this can be found in the large area of the sample, which you will find represented in each image.
Take a look at the figure and you will find that it illustrates the field of view. Everything is done in traditional microscopy methods, and the low magnification effects are visible in each cell. It's in the limited spatial representation that only expands by a few pixels.
When looking at the user interface you will find an alphanumeric display, as well as a numerical keyboard. There are keys of 0-9 for the numbers, and four special keys. You can also find a RUN key and an ON/OFF key on it. When everything is ready you will see the green LED indicator. If at any time the instrument is busy it will show off the color red. Don't worry though; the User Guide offers an explanation on how everything works.
When you're ready to connect the NucleoCounter to a PC you can do so in the USB port. Just make sure this is compatible since the USB ver. 1.1 should be present. Once it's hooked up to the USB port you can use it for transmission of image data. Once this occurs the raw image and processed image are both transferred from the NucleoCounter to the PC. However, analysis must take place within the instrument first. In order for the image transfer to take place the PC needs a special program to make it all happen. You will also find that the raw image and processed image are in the FAQs in the NucleoCounter area, but you can find them here.
The NucleoCounter Instrument is a true stand-alone option that provides all the calculations, cell counting, and data analysis. Keep in mind; the transfer of image data to the PC will only provide additional documentation only.